An Integrated Microfluidic System for Sample Concentration and Extraction
WINNER: 2nd Honorable Mention- Technology to Aid Underserved Populations and Individuals with Disabilities Category
Many patients who are infected with HIV but are on treatment have low viral loads which cannot be detected with conventional qRT-PCRs. The integrated system we have designed concentrates a human plasma sample with defective HIV to make it more easily detectable using less expensive diagnostic techniques. The viral RNA is then extracted using a low-cost solid phase extraction column following virion lysis. qRT-PCR can then be used to measure the viral load from the extracted RNA. This concentration platform improves the limit of detection of conventional qRT-PCR and can be applied in resource limited settings due to its low cost components. This point-of-care concentration device is a step towards a more accessible HIV diagnostic platform.